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Captain Duke Hauser (Channing Tatum) and his G.I. Joe team are set up as traitors and targeted for destruction when the President of the United States (Jonathan Pryce) gives orders to have them terminated, thanks to the influence of Cobra spy Zartan.

But the surviving members of the G.I. Joe team join forces with Joe Colton (Bruce Willis) to bring Zartan down and save the world with their top secret black operation, "Second American Revolution."