Tom Destry (James Stewart), son of a legendary frontier peacekeeper, doesn't believe in gunplay. Thus he becomes the object of widespread ridicule when he rides into the wide-open town of Bottleneck, the personal fiefdom of the crooked Kent (Brian Donlevy).
His detractors laugh even louder when Destry signs on as deputy to drunken sheriff Wash Dimsdale (Charles Winninger). But the laughter subsides when Destry casually proves himself a crack shot, despite his abhorrence of firearms.
Later, when saloon chanteuse Frenchy (Marlene Dietrich), Kent's gal, takes umbrage at Destry's indifferent reaction to her charms, she vows to make a fool of the new deputy. A huge moneymaker, Destry Rides Again served as a spectacular comeback for Marlene Dietrich, who two years earlier had been written off as box office poison.